A New Solution-phase Electrochemical Dna Detection Platform with Target Recycling-based Signal Amplification
نویسندگان
چکیده
We report a rapid, ultrasensitive, immobilization-free and signal-on electrochemical DNA biosensor with excellent discrimination ability for single-nucleotide polymorphisms. It achieves recognition signal generation by the specific cleavage function of exonuclease III and the diffusion different between oligonucleotides and mononucleotides towards a negatively charged ITO electrode surface. Meanwhile, signal can be further amplified by target recycling resulting from the probe digestion process. Combining the advantages of immobilization-free electrochemical detection and isothermal signal amplification strategy, the presented approach may represent a promising path toward direct DNA detection at the point of care.
منابع مشابه
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